Effect of madacassoside on D-galactose-induced learning and memory impairment in mice
10.3969/j.issn.1001-1978.2015.09.012
- VernacularTitle:羟基积雪草苷对 D-半乳糖致亚急性衰老模型小鼠学习记忆的影响及机制研究
- Author:
Shimei TAN
;
Ling WEI
;
Quanfang HUANG
;
Xing LIN
- Publication Type:Journal Article
- Keywords:
hydrocotyle sibthorpioides;
madecasso-side;
D-galactose;
Morris water maze;
Aβ1 -42 proteins;
Aβ-related genes;
synaptic plasticity
- From:
Chinese Pharmacological Bulletin
2015;(9):1239-1243,1244
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect and mechanism of madecassoside from hydrocotyle sibthorpioides (MHS)on learning and memory impair-ment induced by D-galactose (D-gal)in mice.Meth-ods Totally 75 SPF Kunming male mice were divided into normal control group,model group,low-,middle-and high-doses of MHS treated groups.The dementia models were induced with D-gal.The learning and memory functions were tested by Morris water maze, the level of Aβ1 -42 and its related proteins in the hip-pocampus was determined by Western blot,and the ex-pression of Aβ-related genes were determined by RT-PCR.Results Compared with model group,MHS markedly decreased the content of Aβ1 -42 ,inhibited the expression of APP,BACE1 and CatB,but promo-ted the expression of NEP and IDE.In addition,AHS significantly increased the expression of plasticity-relat-ed proteins including PSD-95,p-NMDAR1,p-CaMKII, p-PKACβ,PKCγ,p-CREB and BDNF.Conclusions MHS could remarkably ameliorate the learning and memory impairment induced by D-gal in mice,which may be due to its ability to inhibit the Aβgeneration and deposition and promote synaptic plasticity related protein expression.
