Angiogenesis of protein kinase D1 in bone marrow-derived endothelial progenitor cells of rats
10.3969/j.issn.1001-1978.2015.09.016
- VernacularTitle:蛋白激酶D1在大鼠骨髓源性内皮祖细胞中的促血管新生作用
- Author:
Nuan LIU
;
Lei YANG
;
Bingyu MAO
;
Guochang XU
;
Songshan YE
;
Peihua ZHANG
;
Lifang ZHANG
- Publication Type:Journal Article
- Keywords:
protein kinase D1;
endothelial progenitor cells;
angiogenesis;
endothelial nitric oxide synthase;
migration;
proliferation
- From:
Chinese Pharmacological Bulletin
2015;(9):1259-1263,1264
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of protein kinase D1 (PKD1 )on adherence,migration,proliferation, microvascular formation,and eNOS expression in bone marrow-derived endothelial progenitor cells(EPCs)of rats.Method The EPCs were isolated from bone marrow of rats,cultured and detected,the effects of PKD1 and its specific blocking agent CID755673 on adhesion,migration,proliferation,or microvascular formation were observed,as well as mRNA and protein expression of endothelial nitric oxide synthase (eNOS ) in EPCs.Results PKD1 significantly promoted adhe-sion,migration,proliferation and microvascular forma-tion of EPCs,and upregulated the mRNA and protein expression of eNOS in EPCs,according to the cell cul-ture experiments of EPCs in vitro.Conclusion PKD1 has the role of angiogenesis through regulation of EPCs,which might be dependent on eNOS.
