Preparation and identification of anti-TgAQP peptide antibody
10.3969/j.issn.1002-2694.2015.10.002
- VernacularTitle:抗弓形虫水通道蛋白多肽抗体的制备及鉴定
- Author:
Jiafeng ZHANG
;
Wenbo HAO
;
Bing XIAO
;
Xiaoqing LIAO
;
Shuhong LUO
- Publication Type:Journal Article
- Keywords:
Toxop lasma gondii;
aquaporin;
peptide antibody;
subcellular localization
- From:
Chinese Journal of Zoonoses
2015;(10):903-907,918
- CountryChina
- Language:Chinese
-
Abstract:
In this study ,we intended to prepare anti‐Toxoplasma gondii aquaporin (TgAQP) peptide antibody which was used to the application in the detection of the aquaporin expression and its subcellular localization of Toxop lasma gondii (T .gondii) ME49 strain .The B cell peptide antigen was designed based on the TgAQP amino acids sequence .After the pep‐tide antigen was conjugated to the KLH ,the fusion antigen was injected into New Zealand rabbits to prepare polyclonal anti‐body ,followed by identification of ELISA ,Western‐blotting and immunofluorescence assays .The ELISA showed that the titer of anti‐TgAQP antibody was about 1∶40 000 .Western blotting revealed the specific affinity of the antigen to polyclonal anti‐body at 29 .9 kDa protein T .gondii .The protein detected by the indirect immunofluorescence assays was distributed in the cy‐toplasm of the parasite .Thus far ,the anti‐TgAQP polyclonal antibody was successfully prepared ,providing a useful tool for further study of biological function and metabolic characteristics of TgAQP .
