Determination of Levofloxacin Hydrochloride Ophthalmic in situ Gel by HPLC
- VernacularTitle:HPLC法测定盐酸左氧氟沙星眼用即型凝胶的含量
- Author:
Caixia LIU
;
Qi YANG
;
Aimei DONG
;
Yuren XING
- Publication Type:Journal Article
- Keywords:
Levofloxacin hydrochloride ophthalmic in situ gel;
HPLC;
Levofloxacin
- From:
China Pharmacist
2015;(11):1987-1988,1989
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of levofloxacin in levofloxacin hydrochloride ophthalmic in situ gel. Methods:The chromatographic column was Agilent Zorbax C18(150 mm ×4.6 mm,5μm). The mobile phase consisted of hexane sulfonic acid sodium solution-methanol(72∶28). The flow rate was 1. 0 ml·min-1. The detection wavelength was 293 nm. The chromatographic column temperature was 40℃. The injection volume was 10 μl. Results:The calibration curve was linear within the range of 0. 040 3-0. 403 0 mg·ml-1(r=1. 000 0) for levofloxacin. The average recovery was 99. 8% (RSD=1. 08%,n=9). Conclusion:The method is accurate, simple and rapid, and suitable for the content determination of levofloxacin in levofloxacin hydro-chloride ophthalmic in situ gel.
