A proteomic analysis of effects ofβ-elemene on human gastric cancer cell line SGC7901
10.7652/jdyxb201506025
- VernacularTitle:β-榄香烯对人胃癌细胞 SGC7901作用的蛋白质组学研究
- Author:
Junsong LIU
;
Xiangming CHE
;
Guanglin QIU
;
Lin FAN
;
Wei ZHAO
;
Shicai HE
;
Shuai CHANG
;
Shufeng WANG
- Publication Type:Journal Article
- Keywords:
β-elemene;
gastric cancer;
proliferation;
proteomics;
PAK1IP1;
TOPIIα
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(6):840-844,861
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of β-elemene on SGC7901 gastric cancer cell line and the potential proteins involved. Methods Human SGC7901 gastric cancer cells were treated with different concentrations ofβ-elemene.Cell viability was assessed.A proteomic method,isobaric tags for relative and absolute quantitation (iTRAQ),was employed to detect the proteins altered by β-elemene.Protein expression was validated by Western blot.Results β-elemene inhibited the viability of SGC7901 gastric cancer cells in a dose-dependent manner.Altogether,147 upregulated proteins and 86 downregulated proteins were identified in response to β-elemene treatment in SGC7901 gastric cancer cell line.Among them,the expressions of p21-activated protein kinase-interacting protein 1 (PAK1IP1 ),Bcl-2-associated transcription factor 1 (BTF)and topoisomerase 2-alpha (TOPIIα)were validated by Western blot and the trends were consistent with iTRAQ results.Top pathways involved inβ-elemene treatment in SGC7901 gastric cancer cell line included ribosome signaling,peroxisome proliferator-activated receptors (PPARs)signaling pathway,regulation of actin cytoskeleton,phagosome,biosynthesis and metabolism of some amino acids.Conclusion Our results suggest a promising therapeutic role of β-elemene for gastric cancer.The differentially expressed proteins give us better insights into the potential mechanisms involved in gastric cancer treatment using β-elemene.
