Development of a nested PCR assay for detection of Helicobacter bilis
10.3969/j.issn.1002-2694.2015.10.010
- VernacularTitle:一种检测胆螺杆菌套式 PCR 方法的建立
- Author:
Heping QIN
;
Yong SUN
;
Anli YE
;
Xinyi PAN
- Publication Type:Journal Article
- Keywords:
H .bilis;
nested PCR assay;
16S rRNA
- From:
Chinese Journal of Zoonoses
2015;(10):943-946,951
- CountryChina
- Language:Chinese
-
Abstract:
In this study ,the objective is to establish a nested‐PCR assay for the detection of H .bilis with high sensitivity and specificity .The nested primers were designed based on sequences of 16S rRNA gene of seventeen subtypes of H .bilis .Af‐ter optimizing reaction condition ,the sensitivity and specificity of the assay were examined via the detection of feces simulated samples ,mice infection model samples and clinic patients’ samples .The detection sensitivity of H .bilis strain for feces simu‐lated samples was 10 CFU/100 μL .H .bilis was successfully detected in the liver ,caecum and feces of experimentally infected mice .Moreover ,H .bilis was successfully detected in the bile ,cholecyst mucous membrane and feces samples from two of ten patients with cholelithiasis .Due to the PCR assay’s high sensitivity and specificity ,the method may be used to detect the infec‐tion of H .bilis .
