Effect of TMP combined with cisplatin on expression of Mac2-BP and VEGF in Lewis lung cancer mice
10.7644/j.issn.1674-9960.2015.10.005
- VernacularTitle:川芎嗪联合顺铂对小鼠Lewis肺癌中Mac2-BP和VEGF表达的影响
- Author:
Yafang ZHU
;
Zhihua ZHANG
;
Xiulong ZHANG
;
Zhilin ZHANG
;
Jianhua TANG
- Publication Type:Journal Article
- Keywords:
tetramethylpyrazine;
lung cancer;
angiogenesis;
VEGF;
Mac2-BP/90K/LGALS3BP
- From:
Military Medical Sciences
2015;(10):751-754,764
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tetramethylpyrazine (TMP) combined with cisplatin(DDP) on the expression of Mac2-binding protein(Mac2-BP) and vascular endothelial growth factor (VEGF) in mice with lung cancer. Methods C57 BL/6 mice were subcutaneously inoculated with Lewis lung adenocarcinoma cells , and Lewis lung adenocarcinoma mouse xenograft model was established .Forty mice were randomly divided into four groups:normal saline group(NS group,0.9%NaCl,0.2 ml), TMP group (TMP 100 mg/kg,0.2 ml), DDP group (DDP 2 mg/kg,0.2 ml), TMP plus DDP group (doses as above,0.2 ml totally) with 10 mice in each group.After 2 drug intervention,following 14 days of inoculation , the tumor diameter was measured every two days to calculate the tumor volume .The mice were sacrificed after 14 days of continuous medication , and the subcutaneous tumors were weighed after stripping to calculate the inhibitory rate.The expressions of Mac2-BP and VEGF was detected by Western blot and immunhistochemistry .Results Compared with NS group, the tumor growth rate of TMP group , DDP group and TMP+DDP group was slowed down , and the tumor growth rate in the TMP+DDP group was decreased most significantly .The inhibitory rate of TMP group , DDP group and TMP+DDP group was 25.57%, 45.24% and 66.5%,respectively.Kim, s formula was used to evaluate the synergy of the combined treatment .The q values for TMP +DDP group was 0.85
