Advances in recombinase polymerase amplification
10.7644/j.issn.1674-9960.2015.10.017
- VernacularTitle:重组酶聚合酶扩增技术的研究进展
- Author:
Kui SUN
;
Weiwei XING
;
Donggang XU
- Publication Type:Journal Article
- Keywords:
isothermal nucleic acid amplification techniques;
recombinase polymerase amplification;
lateral flow dip-stick assay;
point-of-care diagnosis;
disease prevention and control
- From:
Military Medical Sciences
2015;(10):802-804,807
- CountryChina
- Language:Chinese
-
Abstract:
Recombinase polymerase amplification (RPA) is a novel isothermal DNA amplification technology first re-ported in 2006 by Piepenburg et al.This technology has been shown to typically work at temperatures ranging from 25 to 43℃and can detect products within 5-20 min.RPA technology requires little instrumentation for the nucleic acid amplifi-cation reaction and can be performed not only in PCR tubes , but also in simple devices′such as paper .Combined with probe-based detection methods or lateral flow dipstick assay , it can perform quantitative or visual detection respectively . RPA is a technology that is potentially ideal for point-of-care diagnosis and disease prevention and control ,characterized by high sensitivity, high efficiency, high specificity and user-friendliness.This paper introduces the advantages and develop-ment of RPA technology in reaction conditions and product detection ,summarizes the current applications of this technolo-gy,and predicts the trend of application of RPA technology in point-of-care diagnosis and disease prevention and control .
