Rsearch of mechanism inhibitory effect on MicroRNA218-Robo1 pathway in breast cancer cell migration

VernacularTitle:MicroRNA218-Robo1通路对乳腺癌细胞迁移的抑制作用和机制的研究
Author: Qiong SONG ; Qimin WANG ; Jieyu MA ; Nai SUN ; Qiu CHEN ; Yanling MA ; Baolin LI
Publication Type:Journal Article
Keywords: Breast neoplasms; MDA-MB-231 cell line; Bioluminescent imaging; MicroRNA
From: International Journal of Surgery 2015;42(9):604-607,封4
CountryChina
Language:Chinese
Abstract: Objective To observe the migration and inhibition mechanism of MicroRNA218-Robo1 pathway for breast cancer.Methods A total of 40 BALB/c-nu/nu female mice were randomly divided into four groups.Each group was transfected over-expression MicroRNA218 MDA-MB-231 breast cancer cells, co-over-expression MicroRNA218 and Robo1 MDA-MB-231 breast cancer cells, knock-down Robo1 MDA-MB-231 breast cancer cells and the control MDA-MB-231 breast cancer cells.The tumor volume was examined every two weeks.Results Tumor volume of MicroRNA218 group was obviously less than control group, tumor volume of Robo1 knock out group was obviously less than common MicroRNA218 high expression and Robo1 group, the difference was statistically significant;MicroRNA218 and Robol knockout group than the control group, the increase in breast cancer cells apoptosis, cell proliferation and angiogenesis is restrained.Conclusions MicroRNA218 inhibited the migration of breast cancer by down-regulating the expression of Robo1.