Histological observation on the effects of TGF-β3 and dental pulp stem cells in repair of rabbit facial nerve injury
10.3760/cma.j.issn.1673-4181.2015.04.008
- VernacularTitle:TGF-β3和DPSCs修复兔面神经损伤的组织学观察
- Author:
Youmei ZHUANG
;
Rong MA
;
Xiaoli ZHANG
;
Hui XU
- Publication Type:Journal Article
- Keywords:
Transforming growth factor-β3;
Facial nerve injury;
Dental pulp stem cells
- From:
International Journal of Biomedical Engineering
2015;38(4):222-224,229,后插7
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of combing the application of transforming growth factor-β3 (TGF-β3) with concentration of 100 ng/μl and dental pulp stem cells (DPSCs) for recovering rabbit facial nerve transverse trauma.Methods Thirty-six healthy adult Zelanian rabbits of clean-grade were selected and randomly divided into group DPSCs+TGF-β3 (experimental group), group TGF-β3 (control group 1) and group PBS (control group 2) with 12 rabbits in each group.The operations for all three groups were applied at rabbit's left cheek.A model of traumatic transection was set on upper buccal branch, then 100 ng/μl TGF-β3 solution and 0.1 ml of 1 ×108/L DPSCs suspension were added into regeneration chamber for the experimental group, while the same amount of 100 ng/μl TGF-β3 solution was added for group TGF-β3 and the same amount of PBS for group PBS.The recovery of facial nerve regeneration with the prepared animal's specimen was evaluated in the 1st, 4th and 12th week after the operation on sacrificed rabbits.Results The effects on nerve regeneration recovery for the experimental group was superior to that of control groups 1 and 2 with all the 36 models included in the result analysis, and that of control group 1 was superior to that of control group 2, which was getting better with the extension of time.Conclusions The combined application of TGF-β3 and DPSCs can effectively promote the facial nerve regeneration, which is better than that of single application of TGF-β3.Meanwhile, the effect with TGFβ3 application is better than that with application of PBS.
