Molecular mechanism for inhibitory effect of puerarin on IL-6 secretion in human osteoblast-like MG-63 cells

VernacularTitle:葛根素抑制人成骨样MG-63细胞分泌IL-6的分子机制研究
Author: Ting LUO ; Lefei LI ; Xiaoqin GUO ; Lipeng ZHANG ; Yue WANG ; Ling LI ; Zemin HAN
Publication Type:Journal Article
Keywords: puerarin; osteoblast; IL-6; ERα; ERβ; siRNA technology
From: Chinese Pharmacological Bulletin 2015;(11):1524-1528,1529
CountryChina
Language:Chinese
Abstract: Aim To investigate the molecular mecha-nism for the inhibitory effect of puerarin on IL-6 secre-tion in human osteoblast-likeMG-63 cells. Methods According to our previous studies, human osteoblast-like MG-63 cells containing two estrogen receptor ( ER) isoforms are a suitable model for this study. En-zyme-linked immnosorbent assay (ELISA), RT-PCR, luciferase reporter assay and small interfering RNA were performed to investigate the effect of puerarin on IL-6 expression in osteoblast-derived cells and underly-ing molecular mechanism. Results Puerarin could obviously inhibit IL-6 expression and IL-6 promoter ac-tivity by human osteoblastic MG-63 cells. Treatment with the ER antagonist ICI 182,780 abrogates the a-bove actions of puerarin on osteoblast-derived cells. U-sing siRNA technology, we further demonstrated that the effects of puerarin on IL-6 production were media-ted by ERα. Conclusion The effect of puerarin on IL-6 production in osteoblast is mediated by ERα.