Ginsenoside F1 induces CYP3 A4 expression through activation of human pregnane X receptor

VernacularTitle:人参皂苷F1通过激活孕烷X受体诱导CYP3 A4的表达
Author: Wenting YOU ; Tao ZHOU ; Zengchun MA ; Qiande LIANG ; Xianglin TANG ; Chengrong XIAO ; Hongling TAN ; Yong XIAO ; Yuguang WANG ; Yue GAO
Publication Type:Journal Article
Keywords: ginsenoside F1; PXR; CYP3 A4; quanti-tative PCR; mRNA; enzyme activity
From: Chinese Pharmacological Bulletin 2015;(11):1536-1539,1540
CountryChina
Language:Chinese
Abstract: Aim To study the effect of ginsenoside F1 on the enzyme activity and expression of gene of CYP3 A4 through activation of pregnane X receptor ( PXR ) . Methods With different concentrations of ginsenoside F1 treated on LS174T cells, the expression of CYP3A4 mRNA was determined by Q-PCR, and the enzyme activity was measured by P450-GloTM CYP3A4 assay according to the manufacturer′s instructions, fur-ther PXR-CYP3 A4 stable translation HepG2 cell lines were used to test ginsenoside F1 activates PXR by re-porter gene screening assay. Results The results re-vealed that the levels of CYP3 A4 gene and protein ex-pression were significantly increased by ginsenoside F1 in a concentration-dependent manner. At the same time, reporter gene screening showed that ginsenoside F1 could also enhance the transcriptional activity of PXR. Conclusion Ginsenoside F1 can significantly up-regulate the gene expression and enzyme activity of CYP3A4 via the PXR-CYP3A4 pathway.