Anticancer activity and mechanism of apoptosis induced by Amaranthus spinosus L. extract in HepG2 cells
10.3969/j.issn.1001-1978.2015.11.016
- VernacularTitle:野苋菜提取物抗肿瘤作用及诱导人肝癌HepG2细胞凋亡的分子机制
- Author:
Jinjuan LIU
;
Chengliang CAO
;
Pan DING
;
Jihong JIANG
- Publication Type:Journal Article
- Keywords:
Amaranthus spinosus L. extract;
anti-cancer;
apoptosis;
caspase-9;
specific inhibitor;
mo-lecular mechanism
- From:
Chinese Pharmacological Bulletin
2015;(11):1558-1561,1562
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the anticancer activity and the mechanism of the apoptosis induced by Ama-ranthus spinosus L. extract ( ASE ) in human hepatic carcinoma cell line HepG2 . Methods Alamar blue assay was used for detecting the influence of ASE on the proliferation of the cancer cells. The morphological changes of cells were observed under inverted micro-scope and Hoechst 33258 stainning. The apoptosis of HepG2 cells was detected by flow cytometry. Western blot and caspase-3 activity kit were used to detect the protein expression in HepG2 cells. The specific inhibi-tor of caspase-9 and caspase-3 ( Z-LEHD-FMK and Ac-DEVD-CHO) was used to validate the signal transduc-tion pathyway. Results The results indicated that the cell proliferation was inhibited by ASE,especicially the HepG2 cells. The HepG2 cells showed obvious apop-totic characteristics. Flow cytometry analysis further validated the apoptosis of HepG2 cells. The expression of Bcl-2 and survivin was downreagulated in HepG2 cells treated with ASE, and Bax, caspase-9, caspase-3, Apaf-1 and PARP were upregualted. Besides, the caspase-3 activity was also increased. Z-LEHD-FMK and Ac-DEVD-CHO significantly increased the cell vi-abilty of HepG2 cells induced by ASE. Conclusion These results confirm that ASE induces the apoptosis of HepG2 through mitochondria-mediated pathway.
