Effect of resveratrol on ROS production and PECAM-1 expression in ox-LDL-stimulated platelets
10.3969/j.issn.1001-1978.2015.11.025
- VernacularTitle:白藜芦醇降低ox-LDL诱导血小板ROS产生和PECAM-1表达的分子机制研究
- Author:
Jie SUN
;
Weijia SUN
;
Beidong CHEN
;
Yanyang ZHAO
;
Li BAO
;
Wei WU
;
Ruomei QI
- Publication Type:Journal Article
- Keywords:
resveratrol;
ox-LDL;
platelets;
ROS;
PE-CAM-1;
Sirt1;
p38 MAPK
- From:
Chinese Pharmacological Bulletin
2015;(11):1608-1613,1614
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of resveratrol on ROS level and PECAM-1 expression in ox-LDL-stimulated platelets. Methods The expression of PE-CAM-1 , Sirt1 and p38 MAPK phosphorylation in ox-LDL-stimulated platelets was determined by Western blot. The level of ROS was measured by immunofluo-rescence kit. Results ox-LDL induced platelet aggre-gation by 14%, whereas resveratrol inhibited platelet aggregation by 50%. Resveratrol decreased ROS level by 3 . 2 fold and completely suppressed PECAM-1 expression in ox-LDL-treated platelets. Resveratrol re-covered Sirt1 expression in ox-LDL-treated platelets. EX527 ( a Sirt1 inhibitor ) increased ROS level and PECAM-1 expression in ox-LDL-stimulated platelets. Meanwhile, resveratrol also suppressed p38MAPK phosphorylation induced by ox-LDL. Conclusion Resveratrol can inhibit platelet aggregation, decrease ROS production and PECAM-1 expression in ox-LDL-stimulated platelets. The mechanism maybe associated with recovery of Sirt1 expression. Moreover, resveratrol can decrease PECAM-1 expression, which may be linked to abolishing p38MAPK phosphorylation.
