Rapid determination of fluoxetine in human plasma by LC-MS/MS
10.3969/j.issn.1673-4130.2015.21.007
- VernacularTitle:LC-M S／M S法快速定量人血浆中氟西汀的浓度
- Author:
Pengfei LI
;
Ping MA
;
Zhuoling AN
;
Cheng WU
;
Ning LEI
;
Weiyue YU
;
Lihong LIU
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
fluoxetine;
pharmacokinetics;
therapeutic drug monitoring
- From:
International Journal of Laboratory Medicine
2015;(21):3092-3094,3097
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish LC‐MS/MS method for the determination of fluoxetine in human plasma .Methods After protein precipitation of acetonitrile the plasma sample was separated on an Agilent XDB‐C18 column using acetonitrile‐1 mmol/L ammonium formate(containing 0 .1% formic acid) as mobile phase by gradient elution .Detection was carried out by multiple reac‐tion monitoring(MRM) on 3200QTRAP LC‐MS/MS system .Results The assay was linear over the range 0 .30 -50 .0 ng/mL with a lower limit of quantitation of 0 .30 ng/mL .Intra‐and inter‐day precision were less than 15% ,respectively .The relative devia‐tion was in the range -2 .80% -2 .09% .The recovery of fluoxetine was more than 98% with less matrix effects .The stabilities were good .Conclusion It could be a rapid ,sensitive ,selective and reliable method for the determination of fluoxetine in human plas‐ma for therapeutic drug monitoring and pharmacokinetics .
