The impact of collagenaseⅡdigestion time on the biological characteristics of rabbit chondrocytes
10.3969/j.issn.1671-8348.2015.32.031
- VernacularTitle:Ⅱ型胶原酶消化时间对兔软骨细胞生物学特性的影响
- Author:
Dechao YUAN
;
Zhu CHEN
;
Shang DENG
;
Gang FENG
- Publication Type:Journal Article
- Keywords:
collagenase;
chondrocytes;
biological characteristics;
cell culture techniques
- From:
Chongqing Medicine
2015;(32):4553-4555,4559
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the best digestion time for rabbit rib cartilage cells separation by collagenase Ⅱ diges‐tion method .Methods Rabbit cartilage tissue was taken from 3‐month‐old New Zealand white rabbits ,and then digested in 0 .2%collagenase Ⅱ solution for 4 ,8 ,12 ,16 and 20 h under vibration at 37 ℃ ,respectively .The corresponding experiments were named as group A ,B ,C ,D ,and E .The obtained primary cells were cultured and passaged after counting .The cell morphology ,proliferation rate ,secretion of extracellular matrix of passage 2 cells were compared to chose the best digestion time .Results The primary chon‐drocytes obtained in group C ,D and E were more than the other two group A and B .The chondrocytes cytoactive in group C and D were better than the other three group A ,B and E .The passage 2 chondrocytes in group C ,D had better secretion of extracellular matrix capability than the other three groups .Conclusion The best collagenase Ⅱ digestion time is 12 to 16 h ,at this digestion time period ,more primary chondrocytes with high cytoactive and strong proliferation capability can be obtained ,and the phenotype of their passage 2 cells is quite good .
