Effect of thymosin beta4 on matrix metalloproteinase-2,9 of hypertrophic scar

VernacularTitle:胸腺素β4对增生性瘢痕基质金属蛋白酶-2及9表达作用的影响
Author: Zhiqiang FAN ; Lixin LIAO ; Youlai ZHANG ; Fei LUO
Publication Type:Journal Article
Keywords: thymosin; matrix; matrix metalloproteinases-2; matrix metalloproteinases-9; extracellular matrix
From: Chongqing Medicine 2015;(31):4321-4323,4326
CountryChina
Language:Chinese
Abstract: Objective To study the effect of thymosin beta4 on the expression of matrix metalloproteinase‐2(MMP‐2) and MMP‐9 of human hypertrophic scar fibroblasts and explore mechanism of thymosin beta4 on hypertrophic scar .Methods Fibro‐blasts were cultured in vitro from the orthopaedic patients(within 6 months after burns) admitted in the first affiliated hospital of Nanchang university from June to September 2013 .The Fibroblasts were divided into experiment group :0 .05 ,0 .1 ,1 .0 μg/mL and 5 .0 μg/mL ,and control group :no thymosin beta4 .The expression levels of MMP‐2 ,MMP‐9 were observed by RT‐PCR and West‐ern blot .Results The expression levels of MMP‐2 and MMP‐9 were increased after the thymosin beta4 took effect ,and it was espe‐cially significant in 1 .0 μg/mL and 5 .0 μg/mL group ,there was statistical difference between experiment group and control group (P<0 .05) .Conclusion Thymosin beta4 can dose‐dependently promote the expression of MMP‐2 and MMP‐9 and inhibit the pro‐liferation of hypertrophic scar .