Application and evaluation of modified sheep blood chocolate culture medium
10.3760/cma.j.issn.1008-6706.2015.23.014
- VernacularTitle:改良羊血巧克力培养基的应用与评价
- Author:
Shiming FU
;
Yueqin SONG
;
Xiangmei ZHU
- Publication Type:Journal Article
- Keywords:
Chocolate medium;
Sheep blood;
Haemophilus influenzae;
Testing techniques and methods
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(23):3564-3565,3566
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the quality and application value of improved sheep blood chocolate medium.Methods The ATCC 10211 of Haemophilus influenzae was inoculated into the modified medium and unmodified medium,the average growth index(GI)of Haemophilus influenzae in two kinds of culture medium was compared.Based on 352 selected qualified sputum specimens for detection of Haemophilus influenzae,the positive isolation rate of medium was compared between the two groups.Results GI value of the traditional blood chocolate culture medium was (3.69 ±0.58),which was significantly lower than (15.08 ±1.34)of the improved sheep blood chocolate culture medium,the difference was significant (t =25.31,P <0.01 ).352 sputum specimens in the improved sheep blood chocolate culture medium,Haemophilus influenzae detected in 41 cases,the positive rate was 11.65%.And 352 sputum specimens in traditional sheep blood chocolate culture,Haemophilus influenzae detected in 18 cases,the positive rate was 5.54%.There was significant differenceof Haemophilus influenzae in separation based on 2 kinds of chocolate culture medium (χ2 =21.04,P <0.05).Conclusion Haemophilus influenzae significantly improved sheep blood colony in chocolate culture medium,grows well,easily to be identified,which helps to detect sputum specimens of Haemophilus influenzae.
