Comparison of body fluid count and classification results detected by Sysmex XE-5000 automatic blood cell analyzer and manual method
10.3969/j.issn.1673-4130.2015.22.007
- VernacularTitle:Sysmex XE-5000全自动血细胞分析仪体液模式与手工法体液细胞计数及分类的比较
- Author:
Hui ZHANG
;
Lin LI
;
Wei XU
- Publication Type:Journal Article
- Keywords:
blood cell analyzer;
manual method;
fluid cell;
cell count;
cell classification
- From:
International Journal of Laboratory Medicine
2015;(22):3238-3239,3242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the body fluid count results detected by Sysmex XE-5000 automatic blood cell analyzer and manual method .Methods A total of 300 cases of body fluid specimens (including cerebrospinal fluid and fluid of serous cavity ) were analyzed .RBC ,WBC counting and classification were respectively detected by XE-5000 and manual method of improvement Neubauer counting plate .Results The fresh specimens without contain a large number of cell clusters ,which RBC counts(RBC-BF)(100-10 000)× 106/L ,and WBC counts(WBC-BF) (9-50)× 106/L ,showed there were a linear relationship between the in-strument method(r=0 .998 5 ,0 .986 3) .In the range ,there was no significant difference between XE-5000 and manual method(t=9 .96 ,P>0 .05) .Also in this range the results of instrument correlated with those of manual method(r= 0 .989 3 ,0 .971 7 , 0 .924 9) .For those specimens which contain a large number of cell clusters ,RBC-BF and WBC-BF were a badly linear relationship between the instrument method(r=0 .564 8 ,0 .456 1) .Conclusion Body fluid specimens which are fresh and do not contain a large number of cell clusters ,in the range of RBC-BF (100 -10 000) × 106/L ,WBC-BF (9 -50) × 106/L ,Sysmex XE-5000 automatic blood cell analyzer could ensure the results have good accuracy .
