Expression of miR-200 a and PTEN in colorectal carcinoma and their clinical significance
10.13315/j.cnki.cjcep.2015.09.010
- VernacularTitle:miR-200 a、PTEN在结直肠癌中的表达及其临床意义
- Author:
Jinglu SUN
;
Hao LI
;
Yu YIN
;
Yijun LI
;
Xian WANG
;
Shan HUANG
;
Yan JIANG
;
Heqin ZHAN
;
Feng YANG
- Publication Type:Journal Article
- Keywords:
colorectal neoplasm;
miR-200a;
PTEN;
in situ hybridization;
immunohistochemistry
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(9):1005-1008,1012
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of miR-200a and PTEN in colorectal carcinoma (CRC) and their relationships with clinicopathologic features. Methods In situ hybridization and immunohistochemistry ( EnVision method) for miR-200a and PTEN were performed in 87 CRCs and normal colorectal tissues distant from tumors. Relationship between expression of miR-200a and PTEN and clinicopathologic parameters of CRC was also analyzed. Results The in situ hybridization showed that the positive expression rate of the miR-200a in CRC was higher than those in normal colorectal mucosa (P<0. 01). The expression of miR-200a was correlated with the degree of tumor differentiation (rs =0. 503, P<0. 01). The immunohistochemistry showed that the positive expression rate of the PTEN in CRC was lower than those in normal colorectal mucosa (P<0. 01). The expression of miR-200a was correlated with the degree of tumor differentiation (rs = -0. 493, P<0. 01). Expression of miR-200a and PTEN was not correlated with age, sex, tumor size, depth of tumor invasion, lymph node metastasis and TNM stage (P>0. 05). The expression of miR-200a had a close negative correlation to that of PTEN in CRC (P<0. 01). Conclusions Overexpression of miR-200a might be associated with the occurrence and development by targeting PTEN, and they could be the indicators in the early diagnosis,treatment and prognosis of CRC.
