Imaging of peripheral tissue perfusion parameters in murine hindlimbs using fluorescence reflectance imaging

VernacularTitle:基于荧光反射成像的小鼠下肢外周组织的灌注参数成像
Author: Simin ZUO ; Fei LIU ; Wei LIU ; Jing BAI ; Jianwen LUO
Publication Type:Journal Article
Keywords: Fluorescence reflectance imaging; Bi-exponential model; Peripheral tissue
From: International Journal of Biomedical Engineering 2015;(3):148-151,155
CountryChina
Language:Chinese
Abstract: Objective To reconstruct the perfusion parameters of murine hindlimbs peripheral tissue by using fluorescence reflectance imaging technique. Methods BALB/c mice were injected with intravenous bolus injection of indocyanine green (ICG) (10μg) into the tail vein. Time-series fluorescence intensity images were obtained for 200 s immediately after the injection. After the serial imaging, silhouette images of the mice were taken under white light to obtain the region of interest (ROI) of the murine hindlimbs. Bi-exponential model was applied to analyze the dynamic fluorescence parameters and the peripheral tissue perfusion parameter images were reconstructed. Results The fitted perfusion curves obtained from bi-exponential model were in good agreement with the measured ones. The parametric images which reflected the vascular sufficiency of murine hindlimbs were reconstructed. Conclusions A novel method for parametric images of murine hindlimbs peripheral tissue blood perfusion is proposed in this paper, which is noninvasive with higher resolution and little damage to biological tissues.