The effect of celecoxib on Wnt/β-catenin signaling pathway and matrix metabolism of chondrocytes in osteoarthritis
10.3760/cma.j.issn.1007-7480.2015.11.008
- VernacularTitle:塞来昔布对骨关节炎中Wnt信号通路和软骨细胞基质代谢的作用
- Author:
Hongyan LIU
;
Yi ZHENG
- Publication Type:Journal Article
- Keywords:
Osteoarthritis;
Wnt signaling pathway;
Metabolism
- From:
Chinese Journal of Rheumatology
2015;19(11):756-760,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study effect of celecoxib on Wnt/β-catenin signaling pathway and matrix metabolism of chondrocytes in osteoarthritis (OA).Methods Use a modified way of mechanical fixation to make rabbit OA model.Knee OA separated and extractd rabbit chondrocytes were and then culture in medium.The cultured chondroctyes were divided into the Dimethyl sulfoxide (DMSO) control group and the celecoxib group, which were given 1％ DMSO and 10 μmol/L celecoxib respectively.After 48 hours' intervention, the protein expression and gene expression of Wnt/β-catenin signaling pathway related factors, including β-catenin,matrix metalloproteinase (MMP)-3, MMP-13, collagen Ⅱ were detected.T-test was used for the comparison between the two groups.Results Compared with the control group, the levels of type Ⅱ collagen [(275±19) ng/ml vs (202±12) ng/ml, t=-6.21, P＜0.05] and proteoglycan [(58±12) ng/ml vs (30±3) ng/ml, t=-9.23, P＜0.05] significantly increased in celecoxib group (P＜0.05).Meanwhile, compared with the control group, the levels of β-catenin, MMP-3, MMP-13 mRNA and protein in celecoxib group were significantly reduced (P＜0.05).Conclusion In rabbit OA chondrocytes, celecoxib can inhibit Wnt/β-catenin signaling pathway, reduce downstream factors expression including MMPs, thereby increase the cartilage matrix type Ⅱ collagen and proteoglycan levels.
