Asiatic acid induces apoptosis in T98 G human glioblastoma cells by changing autophagy
10.3969/j.issn.1001-1978.2015.10.008
- VernacularTitle:积雪草酸通过改变自噬作用促进胶质瘤细胞T98 G凋亡的实验研究
- Author:
Huan LI
;
Fuqin GUAN
;
Yu CHEN
;
Min YIN
;
Hao SUN
;
Ming WANG
;
Xu FENG
;
Yu SHAN
- Publication Type:Journal Article
- Keywords:
asiatic acid;
T98 G cells;
apoptosis;
au-tophagy;
mechanism;
interaction
- From:
Chinese Pharmacological Bulletin
2015;(10):1363-1367,1368
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of asiatic acid on apoptosis and autophagy in human glioblastoma T98G cells. Methods MTT colorimetry was employed to assay the cellular proliferating activity. The fluores-cence microscope and Hoechst 33258 staining were used to detect the morphological changes. The cell ap-optosis and autophagy were analyzed by flow cytometry with Annexin-V/7-AAD and MDC staining respective-ly. The expressions of associated proteins were detected by Western blot to analyze the mechanism of apoptosis and autophagy. Results MTT assay showed that the growth of T 9 8 G cells was inhibited by asiatic acid ( IC50 =46. 3 μmol · L-1 ) . Annexin V/7-AAD stai-ning and Western blot revealed that asiatic acid in-duced apoptosis in T98 G cells by reducing the expres-sion of Akt, decreasing the mitochondrial membrane potential, and increasing the expression of Caspase-3. MDC staining and Western blot showed that the per-centage of MDC-positive cells was decreased and the expressions of Beclin-1 , LC3-II and Atgs were inhibi-ted by asiatic acid treatment. 5 μmol·L-1 chloroquine was used to up-regulate the expressions of LC3-Ⅱand Beclin-1 . Asiatic acid-inhibited autophagy was blocked and the total apoptotic rate was reduced remarkably. Conclusion Asiatic acid suppresses T98 G cells pro-liferation by inducing apoptosis and inhibiting cell au-tophagy, and the very role of inhibiting autophagy could promote apoptosis to a certain extent.
