The contrast research and application of electrochemiluminescence immuno-assay determination kits of human embryonic antigen
10.3969/j.issn.1673-4130.2015.19.034
- VernacularTitle:人癌胚抗原电化学发光免疫分析测定试剂盒的对比研究及应用?
- Author:
Zhong LI
;
Xiaoming HE
;
Peimin LI
;
Lixian WEN
;
Ya LI
;
Youhai PENG
;
Haiying GAN
- Publication Type:Journal Article
- Keywords:
carcinoembryonic antigen;
electrochemiluminescence immunoassay;
imported reagents
- From:
International Journal of Laboratory Medicine
2015;(19):2852-2853,2856
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the comparability of test results of self-built human carcinoembryonic antigen(CEA)electro-chemiluminescence immunoassay(ECLIA)and imported reagent.Methods A total of different concentrations 77 fresh serum speci-mens were collected and detected CEA by two kinds of ECLIA kit.The results were analyzed with Excel2003 and SPSS1 9.0 soft-ware.Results The difference between each dose was significant (P <0.05),and the detection results between each had no signifi-cant difference (P >0.05);the sensitivity of the assay was 0.3 ng/mL,the intra coefficient of variation was 4.58%-5.83%,the inter coefficient of variation was 5.07%-5.97%,the analytical recovery was 99.13%-107.28%,the specificity of the assay had no cross reaction with CA1 99 and AFP.The correlation coefficient between two kinds of reagents determination results was greater than 0.95,with imported reagent as reference test,self-built carcinoembryonic antigen ECLIA clinical performance evaluation was acceptable.Conclusion The precision of the two kinds of ECLIA in detection of CEA accord to clinical requirement.Comparability exists in evaluating the acceptability of clinical.
