N-cadherin regulates projection of spinal commissural axons viaβ-catenin during chicken embryonic development
10.3969/j.issn.1000-484X.2015.10.013
- VernacularTitle:N－cadherin通过β－catenin调控鸡胚脊髓连合纤维向对侧的投射
- Author:
Ciqing YANG
;
Xiaoying LI
;
Congrui WANG
;
Bichao ZHANG
;
Han LI
;
Juntang LIN
- Publication Type:Journal Article
- Keywords:
Chicken embryonic;
Neural cadherin;
β-catenin;
Nerve fibers
- From:
Chinese Journal of Immunology
2015;(10):1357-1360,1374
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of N-cadherin and β-catenin in the formation of spinal commissural axon projection during chicken embryonic development.Methods:Fertilized eggs were cultured for three days(stage22),N-cadherin orβ-catenin inter-ference plasmid was injected into the neural tube and in vivo electroporation was performed.Three days after the electroporation, embryos were collected,fixed with 4%PFA,embeded with OCT,and cut into frozen sections.Four groups ( knockdown of N-cadherin orβ-catenin or both of them,and control) were included in this study.Immunohistochemistry method was used to analyze the protein expression result of N-cadherin or β-catenin.The changes of spinal commissural projections were observed with GFP fluorescence.Results:During chicken embryonic development,knockdown of N-cadherin inhibited the expression of β-catenin in the spinal cord.The commissural nerve fibers projecting to the contralateral side of the spinal cord was impaired after knockdown of N-cadherin or β-catenin;this phenotype was similar after knocking down both of them.Conclusion: N-cadherin is implicated in the formation of spinal commissural projection in the developing spinal cord,possibly viaβ-catenin.
