Synthesis and biocompatibility of ethynylated open ring derivatives of polyasparamide
- VernacularTitle:炔基化聚天冬酰胺开环衍生物的合成及生物相容性研究
- Author:
Honglin GAO
;
Jinghua HAN
;
Cuihong YANG
;
Yajie LIU
;
Naling SONG
;
Yanming WANG
- Publication Type:Journal Article
- Keywords:
derivatives of polyasparamide;
ethynylation;
drug carrier;
biocompatibility
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(9):1-3,7
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize a new ethynylated open ring derivatives of polyasparamide as functional drug carrier.Methods L-phenylalanine methyl ester hydrochloride was prepared using L-phenylalanine and then was used for ring opening reaction of polysuccinimide.To synthesize the target product of PSI-Phe-OMe-PA, the obtained polyasparamide-g-phenylalanine derivatives ( PSI-Phe-OMe) was further ring opened by propargylamine.The structure of PSI-Phe-OMe-PA was confirmed by 1 H NMR.The biocompatibility of PSI-Phe-OMe-PA was evaluated by MTT method, inverted microscope observation and cell cycles analysis ( propidium iodide staining ) .Results The ring-opening rate of polyasparamide by L-phenylalanine methyl ester and propargylamine was 40%and 100%, respectively.All results of biocompatibility studies indicated that PSI-Phe-OMe-PA may be a good candidate for functional drug carrier.Conclusion Based on the ring-opening capability of amino-group and the specificity of click reaction, L-phenylalanine methyl ester hydrochloride and propargylamine were used successively to react with polyasparamide.PSI-Phe-OMe-PA is a biocompatible functional drug carrier.
