Levels of autophagy in T cells and B cell of patients with systemic lupus erythe-matosus and its clinical significance
10.3969/j.issn.1000-484X.2015.10.018
- VernacularTitle:系统性红斑狼疮患者外周血T、B细胞自噬水平及其临床意义
- Author:
Yanwen CHEN
;
Chao WANG
;
Xuguang WANG
;
Yongmin FENG
;
Ning AN
;
Hongluan WU
;
Shangmei LI
;
Jingli TAO
;
Weijing LIU
;
Huafeng LIU
;
Qingjun PAN
- Publication Type:Journal Article
- Keywords:
Systemic lupus erythematous;
T lymphocytes;
B lymphocytes;
Autophagy;
LC3-Ⅱ
- From:
Chinese Journal of Immunology
2015;(10):1380-1384,1388
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate levels of autophagy in T cells and B cell of patients with systemic lupus erythematosus ( SLE) and its clinical significance.Methods: 68 SLE patients without treatment within 4 weeks were enrolled in this study.We accessed the levels of autophagy in T cells and B cells of 23 healthy controls and 68 patients before and after treatment by flow cytometry,and analyzed their correlations with serum levels of C3 and anti-dsDNA antibodies,SLEDAI score,et al.Results: Before treatment,a significantly increased levels of LC3-Ⅱ was observed in SLE patients than healthy controls, the active group ( SLEDAI score≥10) was significantly higher than the stable group(SLEDAI score<10),and the newly diagnosed group was significantly higher than the recurrent group(all P<0.05).While the levels of LC3-Ⅱ was decreased in B lymphocytes in SLE patients,the active group was significantly lower than stable group,and the active group was significantly lower than the newly diagnosed group(all P<0.05). Correlation analysis found that,a positively correlation was observed for the levels of LC3-Ⅱwith SLEDAI score in T lymphocyte( rs=0.289,P<0.05),and the levels of C3 were positively correlated the levels of LC3-Ⅱ in B lymphocyte(rs=0.371,P<0.01).After treatment for five days, levels of autophagy in T lymphocytes of SLE patients with good prognosis ( SLEDAI score decreased ≥4 ) significantly decreased(P<0.05).Also,three days after treatment,levels of autophagy in B lymphocytes of SLE patients with good prognosis were increased significantly ( P<0.05 ) .However, SLE patients with poor prognosis had no such difference ( P>0.05 ) . Conclusion:Levels of autophagy in T and B lymphocytes of SLE patients are abnormal compared to healthy controls,and these changes are associated with disease activity.Also,these changes are expected to be the indicators of disease activity and potential therapeutic targets in SLE.
