Long-term effects of different drugs on contralateral testis with unilateral testicular torsion in prepubertal rats
10.3969/j.issn.1000-4718.2015.08.027
- VernacularTitle:不同药物对青春前期大鼠睾丸扭转复位健侧睾丸的远期影响
- Author:
Jie YANG
;
Jianhua ZHANG
;
Fei YANG
;
Lei QIU
;
Jie JIAN
;
Fang JIE
;
Jinping ZHANG
- Publication Type:Journal Article
- Keywords:
Testiculartorsion;
Oxygenfreeradical;
Taurine;
Salviamiltiorrhiza;
Xueshuantong;
Brevisca-pine;
Prepuberty
- From:
Chinese Journal of Pathophysiology
2015;(8):1500-1504,1509
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]AIM:Toobservethelong-termeffectsoftaurine,Danxuetong(Salviamiltiorrhizacombinedwith Xueshuantong ) and breviscapine on contralateral testis with unilateral testicular torsion in prepubertal rats , and to compare their protective effect on testis with ischemia reperfusion injury .METHODS: Four-week-old healthy male SD rats ( n=64) were randomly divided into 8 groups:sham operation group, saline group, single-dose and successive taurine groups, single-dose and successive Danxuetong groups , and single-dose and successive breviscapine groups .The animal model of left testicular torsion reduction (720°for 2 h) was established.After 6 weeks of surgery, the right testes and epididymides from the rats were collected immediately to detect the total antioxidant capacity (T-AOC), the activity of superoxide dis-mutase (SOD), the activity of nitric oxide synthase (NOS) and the content of malondialdehyde (MDA).The sperm de-formity, sperm viability and sperm concentration were measured , and the pathological examination of the testicular tissues was performed.RESULTS:Compared with saline group, the activity of SOD, T-AOC, sperm viability and sperm concen-tration were increased , while MDA content and sperm deformity were decreased in 3 successive groups .SOD activity and sperm viability ( except single-dose breviscapine group ) were increased , but MDA content and sperm deformity were de-creased in 3 single-dose groups .T-AOC activity and sperm concentration were increased in single-dose taurine group .The saline group showed interstitial edema and degeneration of seminiferous tubules , and the rest groups showed a significant improvement in histological morphology .CONCLUSION:The contralateral testis in prepubertal rats was damaged after u-nilateral testicular torsion , and taurine , Danxuetong and breviscapine have long-term protective effect on the tissues under ischemia-reperfusion injury .The protective effect of Danxuetong is better than breviscapine , and taurine is the best of them.The effect of single-dose group is better than the successive application .
