Effect of sitagliptin on the expression of NO/iNOS in type 2 diabetic nephropathy
- VernacularTitle:西格列汀对2型糖尿病大鼠肾组织中NO／iNOS水平的影响
- Author:
Dongdong WANG
;
Tong WEI
;
Sumei HE
;
Guanying ZHANG
;
Di YIN
;
Qunli WEI
- Publication Type:Journal Article
- Keywords:
sitagliptin;
type 2 diabetic kidney;
inducible nitric oxide synthase;
nitric oxide
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(7):10-12,16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect of sitagliptin ( SIT) on the expression of inducible nitric oxide synthase ( iNOS) and nitric oxide ( NO) in type 2 diabetic nephropathy.Methods 30 rats were randomly divided into normal group (NC group), diabetic nephropathy group (DN group) and sitagliptin treatment group (SIT group).The type 2 diabetes mellitus (T2DM) rats were induced by a high fat diet (HFD) plus repeated low dose streptozocin (STZ) injections.At the end of the 12th week in treatment,there were 6 rats in each group, the NO level was determined by Griess method.mRNA levels of iNOS RT-PCR was detect ed by.The expression of iNOS protein was detected by western blot and immunohistochemical method. ResuIts Compared with the NC group, the expression of iNOS and NO of DN group increased significantly.However, compared with DN group, the expression of iNOS and NO of SIT group decreased significantly.ConcIusion SIT can decrease the expression of iNOS and NO, which implies SIT may protect the type 2 diabetic kidney.
