Protective effect of NADPH oxidase against cerebral ischemia/reperfusion injury
10.3969/j.issn.1001-1978.2015.08.020
- VernacularTitle:抑制NADPH氧化酶对脑缺血/再灌注损伤的保护作用
- Author:
Li YU
;
Xuhui TONG
;
Zongbing FAN
;
Yinling CHEN
;
Yan LI
;
Shuying DONG
- Publication Type:Journal Article
- Keywords:
cerebral ischemia/reperfusion injury;
NADPH oxidase;
apocynin;
Cx36;
PKC;
apoptosis
- From:
Chinese Pharmacological Bulletin
2015;(8):1126-1130,1131
- CountryChina
- Language:Chinese
-
Abstract:
Aim To determine the protective effect of NADPH oxidase against focal cerebral ischemia/reper-fusion ( I/R) injury in rats. Method A thread occlu-sion method was used to make a middle cerebral artery occlusion ( MCAO ) model. Apocynin ( 2. 5 mg · kg-1 ) was injected by tail vein 15 min before ischemi-a. Then, the neurological behavior, cerebral infarction volume, pathological morphological changes and the expression of Cx36, PKC, Bax, Bcl-2 of rats were de-tected after ischemia for 2 h, followed by reperfusion for 24 h. Results Compared with cerebral I/R group, administration of apocynin significantly reduced the neurological behavior scores and the cerebral in-farction volume percentage, alleviated the pathological morphological damage, increased the protein expres-sion of Cx36 and PKC, and reduced the Bax/Bcl-2 ra-tio of rats with focal cerebral I/R injury. Compared with apocynin group , apocynin combined with PKC inhibitor significantly reduced above protective effects. Conclusion Inhibition of NADPH oxidase could alle-viate cerebral I/R injury, increase the levels of Cx36, PKC proteins and reduce the Bax/Bcl-2 ratio.
