Effect of ionizing radiation on bone marrow derived c-kit+ cells in vitro
10.3969/j.issn.1671-8348.2015.24.001
- VernacularTitle:电离辐射对小鼠骨髓 c-kit 阳性细胞损伤效应体外研究
- Author:
Junling ZHANG
;
Bing LIU
;
Lu LU
;
Deguan LI
;
Aimin MENG
- Publication Type:Journal Article
- Keywords:
bone marrow;
radiation,ionizing;
radiation injuries;
c-kit+ cells
- From:
Chongqing Medicine
2015;(24):3313-3315,3318
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the injury effect of ionizing radiation on bone marrow derived c-kit+ cells.Methods Via-bility of c-kit+ cells was measured by bioluminescence;the level of c-kit+ cells reactive oxygen species was measured by DCFH-DA, the ability of colony-forming units was reflected by CFU-GM;proliferation and apoptosis of c-kit+ cells were measured by flow cy-tometry;the variation of pathway was detected by arrays of gene chip.Results Compared to control group(0 Gy).It had a decrease of c-kit+ cells′cell viability and the ability of colony-forming units after the cells receipt irradiation with the dose of 1 Gy and 4 Gy;and the level of cell reactive oxygen species,ratio of apoptosis cells increased.After 1 Gy irradiation exposure,the ratio of prolifera-tion(S/G2/M phase)cells increased compared to control group.However,when the c-kit+ cells were receipt 4 Gy irradiation expo-sure,the ratio of proliferation(S/G2/M phase)cells decreased.After 4 Gy irradiation exposure,the up-regulate genes contained Srxn1,Psmb5,Cdkn1a,Smc1b,Bcl2l1,Lrdd and so on;the down-regulate genes contained Mpo,Mtf1,Chek1,Rcc1 Ebag9,Ciapin1 and so on.Conclusion There was injury effect of ionizing radiation on c-kit+ cells,and it could induce variation of many pathways.
