Activating Sonic hedgehog pathway can improve the impaired function of endothelial progenitor cells in type 1 diabetic mice
10.3969/j.issn.1001-1978.2015.08.008
- VernacularTitle:激活Sonic hedgehog 通路改善1型糖尿病小鼠内皮祖细胞功能
- Author:
Yuan QIN
;
Yanhua HE
;
Genshui ZHANG
;
Guiping ZHANG
;
Jiandong LUO
- Publication Type:Journal Article
- Keywords:
endothelial progenitor cells;
type 1 diabe-tes mellitus;
Sonic hedgehog pathway;
proliferation;
migration;
tube formation;
senescence
- From:
Chinese Pharmacological Bulletin
2015;(8):1066-1070,1071
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of activating Sonic hedgehog( Shh) pathway on the function of endothelial progenitor cells ( EPCs ) in type 1 diabetic mice. Methods EPCs were isolated and cultured by density gradient method from diabetic mice. The effects of Shh N-terminal peptide and agonist SAG on EPCs prolifera-tion were evaluated by using the MTT colorimetric as-say. EPCs migration was measured by Transwell meth-od. EPCs tube formation ability was estimated by Matrigel . EPCs senescence activity was determined by β-galactosidase staining. Results Compared with control mice, the function of EPCs in type 1 diabetic mice was impaired. The proliferation, migration and tube formation of diabetic EPCs could be promoted by Shh peptide and agonist SAG. The senescence of dia-betic EPCs could be decreased by Shh peptide and ag-onist SAG. Conclusion Activating Shh signaling pathway can improve the impared function of diabetic EPCs in type 1 diabetic mice.
