PGE1 alleviated alveolar cells apoptosis in rat lung impact injury model
10.3969/j.issn.1671-8348.2015.23.006
- VernacularTitle:前列腺素 E1减轻大鼠肺撞击伤后肺泡细胞凋亡?
- Author:
Fuchun LIU
;
Yi HUANG
;
Xuecheng LI
;
Yanmei LUO
;
Jun GAO
;
Hao ZHANG
- Publication Type:Journal Article
- Keywords:
prostaglandins;
apoptosis;
autophagy;
alveolar cells;
NIX
- From:
Chongqing Medicine
2015;(23):3187-3189,3194
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of prostaglandin E1 (PGE1)on alveolar cells apoptosis in rat lung impact in-jury model.Methods SD rats were divided into 3 groups (normal control group,lung injury control group and PGE1 treated group).PaO2 and pulmonary coefficient were detected after 24 h of impact.TUNEL labeling was used to evaluate apoptosis and Western blot was used to estimate protein expression levels of beclin-1,LC3 Ⅱ/LC3 Ⅰand NIX.Results After 24 h of impacting, there were obvious structural damage and pneumonedema in rat lung.Compared to normal control group,the PaO2 of lung injury control group decreased and the apoptosis of alveolar cells increased significantly(P <0.05).Furthermore,the expression levels of Beclin-1,LC3Ⅱ/LC3Ⅰ and NIX in the impacting control group were increased (P <0.05 ).In the PGE1 treated group,the PaO2 were decreased compared to normal control group(P <0.05),but these expression levels were higher significantly than lung injury control group (P <0.05).The expression levels of apoptosis,Beclin-1,LC3Ⅱ/LC3Ⅰ and NIX in the PGE1 treated group were in-creased compared to normal control group(P <0.05),but these expression levels were lower significantly than lung injury control group (P <0.05).Conclusion PGE1 could alleviate alveolar cells apoptosis after lung impacting injury,and which effect may as-cribe to PGE1 inhibiting NIX-mediated autophagy and autophagic apoptosis of alveolar cells.
