The gene expression of oral squamous cells carcinomas and buccal mucosa premalignant lesions and the research on the difference of cellular pathways
10.3969/j.issn.1671-8348.2015.23.004
- VernacularTitle:口腔鳞状细胞癌和颊黏膜癌前病变基因表达和细胞通路的差异性研究?
- Author:
Fujun ZHANG
;
Guodong ZHANG
;
Kai YANG
;
Jie MEI
- Publication Type:Journal Article
- Keywords:
oral;
precancerous lesion;
neoplasms,squamous cell;
gene;
pathway
- From:
Chongqing Medicine
2015;(23):3180-3182,3186
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and analysis the virulent genes and pathways in golden hamster cheek pouch mucosa precan-cerous lesions and squamous cell carcinomas.Methods The experimental models of golden hamster cheek pouch mucosa precancer-ous lesions and squamous cell carcinomas were induced by DMBA.The total RNA of precancerous lesions and squamous cell carci-nomas of golden hamster cheek pouch was extracted and the cRNA was labeled by Cy3.Then gene chip was used to screen the dif-ferentially expressed genes.At last,the Gene Ontology and pathway was used to analysis the biology function of important virulent genes.Meanwhile,we confirmed the correctness of the results by using the RT-PCR.Results A total of 1 981 differentially ex-pressed genes were detected during the process from precancerous lesions to squamous cell carcinomas (120 genes remained known).One thousand and thirty-seven genes were up-regulated and 944 genes down-regulated.GO analysis showed that these dif-ferentially expressed genes mainly related to the macromolecular metabolism,signal transduction and so on.Pathway analysis showed that 9 pathways were significant changes.14 genes were enriched in above 9 change pathways.Conclusion There were 1 981 differentially expressed genes and 9 abnormal changes pathways during the process from precancerous lesions to squamous cell carcinomas,in which 14 differentially expressed genes led to changes in cellular pathways.These genes might be likely to have the important pathogenic genes in the process of transformation.
