ALKBH5 inhibits proliferation of HepG2 and L-02 cells
10.7644/j.issn.1674-9960.2015.08.005
- VernacularTitle:ALKBH5抑制肝癌细胞系HepG2和肝细胞系L－02的增殖能力
- Author:
Ranran MENG
;
Ying ZHANG
;
Gangqiao ZHOU
- Publication Type:Journal Article
- Keywords:
ALKBH5;
liver cancer;
cell proliferation
- From:
Military Medical Sciences
2015;(8):593-596,601
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of AlkB homologue 5 ( ALKBH5 ) on proliferation, cell cycle, and apoptosis of HepG2 and L-02 cells.Methods Recombinant plasmid vector containing the CDS region of ALKBH5 (pEGFP-C1b-ALKBH5) was stably transfected into HepG2 and L-02 cells.Western blotting was used to detect the expression of green fluonescence protein ( GFP )-ALKBH5.There were two groups in our experiment: GFP-ALKBH5 lentivirus group and GFP lentivirus group.Characteristics, such as proliferation, cell cycle and apoptosis of HepG2 and L-02,were detected through Cell Counting Kit-8 (CCK-8) assay, flow cytometry and clone formation, respectively.Results The result of Western blotting revealed that ALKBH5 was efficiently up-regulated at protein levels.Despite apoptosis, phenotypic analysis revealed that the proliferation and cell phases were significantly inhibited in ALKBH5 overexpressed stable cell strains compared with the control cells (both P<0.05).Conclusion ALKBH5 can restrain fetal liver cell (L-02) and hepatocellular carcinoma cell (HepG2) from proliferating.Taken together, our results strongly suggest that ALKBH5 can play a key role in the generation and progression in HCC as a tumor suppressor.
