Innate immunology research of glutaraldehyde-treated xenogenic blood vessels
10.3969/j.issn.1000-484X.2015.08.019
- VernacularTitle:戊二醛固定的异种血管的先天性免疫机制研究
- Author:
Ying ZHANG
;
Da GONG
;
Yi XIN
;
Wei CUI
;
Jielin LIU
;
Juyi WAN
;
Sa LIU
;
Diankun LI
;
Lanping DU
;
Zhifei XIN
;
Xiufang XU
;
Wenbin LI
- Publication Type:Journal Article
- Keywords:
Rat subcutaneous embedding experiment;
Xenogenic transplantation rejection;
Immunohistochemistry;
Innate im-munology
- From:
Chinese Journal of Immunology
2015;(8):1085-1088,1093
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To conduct a systematic study of the immunologic response of rats to transplanted glutaraldehyde ( GA)-treated porcine blood vessels in vivo.Methods: The experiment was divided into two groups:fresh group and glutaraldehyde-treated group.Twenty cases of fresh and glutaraldehyde-treated porcine pulmonary arteries were subcutaneously embedded in rats.We compared the changes using HE staining and immunohistochemistry.Results:HE staining showed that there were stronger expression on day 12 and day 30 in the fresh group than that in the glutaraldehyde group.There were similar results in morphology in CD68,C3,IgG.The results of integral optical density ( IOD) in immunohistochemistry showed that IOD started rising from day 4 and got the peak on day 12 or day 30 and or fell on day 60.Conclusion: Innate immunity played an important role in the research on xenogenic immunological rejection mechanism.The immunogenicity of glutaraldehyde-treated xenogenic blood vessels is lower than that in fresh blood vessels.However there is still immunogenicity in glutaraldehyde-treated xenogenic blood vessels.We will explore better ways to obviously weaken the rejection.
