Preliminary mechanism of edaravone against cell apoptosis after spinal cord injury in rats
10.11958/j.issn.0253-9896.2015.09.008
- VernacularTitle:依达拉奉抗大鼠脊髓损伤后脊髓细胞凋亡的机制初探
- Author:
Jiquan WANG
;
Xingchang ZHAO
;
Ping SUN
;
Haotian LI
;
Xin CHU
;
Gang LYU
;
Zhongkai FAN
- Publication Type:Journal Article
- Keywords:
spinal cord injury;
apoptosis;
caspase 3;
caspase 12;
models,animal;
rats,Sprague-Dawley;
endoplasmic reticulum stress;
edaravone;
transcription factor CHOP
- From:
Tianjin Medical Journal
2015;(9):988-991,1092
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of edaravone (EDA) on cell apoptosis induced by endoplasmic reticu?lum stress (ESR) after spinal cord injury (SCI) in rats. Methods Thirty-six healthy adult SD rats were randomly divided in?to three groups (12 rats for each group):Sham group, SCI group and EDA group. The rat model of SCI was made by Allen’s method and the sham group was only received laminectomy and kept the spinal cord intact. Rats in sham group and SCI group accepted the same volume and frequency of saline injection as EDA group. The EDA group was given 10 mg/kg EDA once every 12 h intraperitoneally. Three days after injuring, the spinal cords were harvested, and the protein levels of C/EBP homologous protein (CHOP), Cleaved caspase-12 and Cleaved caspase-3 were detected by Western blot assay. Immunofluo?rescence staining was used to analyze the positive ratio of caspase-12 and CHOP in spinal cord of three groups. Meanwhile, TUNEL staining was used to identify cell apoptosis of spinal cord. Results Compared with sham group, the protein levels of CHOP, Cleaved caspase-12 and Cleaved caspase-3 were obviously higher in SCI group (P<0.01);the proportion of Cas?pase-12 and CHOP positive cells was significantly increased (P<0.01), and the apoptotic rates were also significantly in?creased in spinal cord (P<0.01). However, compared with SCI group, the protein levels of CHOP , Cleaved caspase-12 and Cleaved caspase-3 were significantly decreased in EDA group (P<0.01);the proportion of Caspase-12 and CHOP positive cells was significantly reduced (P<0.01), and the apoptotic rates were also significantly decreased in spinal cord (P<0.01). Conclusion EDA has neuroprotective potential to spinal cord injury. The mechanism of its neuroprotective effect may asso?ciate with its inhibitory effect to the cell apoptosis induced by endoplasmic reticulum stress after SCI.
