Clinical application and comparison of rapid and accurate identification of mycobacterium by gene chip microarray and smear acid-fast staining
10.3969/j.issn.1673-4130.2015.16.018
- VernacularTitle:基因芯片快速分枝杆菌鉴定技术与涂片抗酸染色技术的临床应用比较?
- Author:
Hu HOU
;
Aimin LI
;
Shuming TANG
- Publication Type:Journal Article
- Keywords:
gene chip;
smear acid-fast staining;
mycobacterium
- From:
International Journal of Laboratory Medicine
2015;(16):2335-2336,2338
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the clinical effect of application of gene chip microscopy technique for rapid identification of Mycobacterium and classic smear acid-fast staining,and to assess the advantages and disadvantages of the wo methods.Methods From 201 1 to 2014,gene chip microarray and smear acid fast staining were used to identify the mycobacterium tuberculosis in speci-mens suspicious of the infection from all the general hospitals of Shenzhen city.Chi-square test was used to compare the positive rates of the two methods.Results A total of 2 481 specimens were collected from clinic.With smear acid-fast staining technique, the positive specimens of 1 93 cases werefound and the positive rate was 7.8%.Meanwhile,31 7 positive samples were detected by the technology of gene chip microarray,and the positive rate was 12.8%.The positive rate of Gene chip microarray technology was higher than that of the smear acid fast staining,and there was significant difference between them (P < 0.05 ).The 31 7 positive samples identified by Gene chip microarray,included 263 cases of Mycobacterium tuberculosis,27 cases of Mycobacterium absces-sus,18 cases of Mycobacterium intracellulare,3 cases of Mycobacterium gastric uLcer,3 cases of Mycobacterium avium,1 case of Mycobacterium Gordonae,1 case of Mycobacterium marinum and 1 case of Mycobacterium Kansas.Conclusion The gene chip mi-croarray technology is fast,accurate,and its positive rate is higher than that of smear acid-fast staining technique.Classification and identification of Mycobacterium is very helpful for clinical individualized treatment of anti mycobacterium infection.
