Comparison Analysis of the Results of Serum Anti CCP Antibody in Patients with RA by Two Methods
10.3969/j.issn.1671-7414.2015.03.032
- VernacularTitle:两种方法检测 RA 患者血清抗 CCP 抗体的结果比对分析
- Author:
Yahong MU
;
Xiaoling LIU
;
Huixia BAI
;
Mengtao WANG
;
Hongyi LIU
;
Min GAO
;
Lina TIAN
;
Jianfeng ZHOU
;
Zhijuan YAO
- Publication Type:Journal Article
- Keywords:
anti CCP antibody;
ELISA;
latex turbidimetric method
- From:
Journal of Modern Laboratory Medicine
2015;(3):111-112,114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare diagnosis value and the clinical application of enzyme linked immunosorbent assay (ELISA) method and the immune turbidimetric method detecting serum anti cyclic citrullinated peptide (CCP)antibody in patients with RA.Methods Collected fresh serum specimen of 267 inpatients with RA in Rrheumatism Department of Xi’an Institu-te of Rheumatism from December 2014 to February 2015,and fresh serum specimen of 50 healthy blood donors from the Blood Center of Shaanxi Province respectively.Anti CCP antibody was detected by enzyme-linked immunosorbent assay (ELISA)method and the latex immunoturbidimetry assay.Evaluated the correlation of the results and clinical application to RA diagnosis.Results Sensitivity,specificity and diagnostic consistency of ELISA and latex immunoturbidimetry assay were 77.3%,86.8%,94.3% and 76.2%,80.2%,77.9% respectively.Compared two kinds of methods,the value of Kappa was 0.756,for having consistency.Throughχ2 test (χ2 =1.85,P >0.05),there was no significant difference between two meth-ods.Area of ELISA and lateximmunoturbidimetry under the ROC curve were 0.876 and 0.832 respectively.Conclusion De-tection of serum anti CCP antibody has diagnostic value in RA patients.The ELISA method and the latex immunoturbidime-try assay for detection of anti CCP antibodies had consistency.Two methods had no statistical difference,and the latex turbi-dimetric method is suitable for grassroots medical institutions.
