Expression and Clinical Significance of Bcl-6 mRNA in the T Follicular Helper(Tfh)Cells of HIV/AIDS Patients
10.3969/j.issn.1671-7414.2015.03.018
- VernacularTitle:Bcl-6mRNA在HIV／AIDS患者滤泡辅助性T细胞中的表达及意义
- Author:
Qian FENG
;
Weihong REN
;
Yanqing LI
;
Wenbo LI
;
Yan TONG
;
Hang ZHAO
;
Huijie FENG
- Publication Type:Journal Article
- Keywords:
Bcl-6;
Tfh cells;
HIV;
B cells
- From:
Journal of Modern Laboratory Medicine
2015;(3):64-66,70
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of Bcl-6 mRNA in the Tfh cells of HIV/AIDS patients and the relationship between Bcl-6 mRNA and the progression of HIV/AIDS.Methods This experiment chose 60 patients who were confirmed by HIV antibody test positive,during May 2014 to November from AIDS Research Institute in the First Affiliated Hospi-tal,Henan College of Traditional Chinese Medicine.According to the amount of CD4+T cells,the patients with HIV/AIDS were divided into A(CD4<200 cells/μl),B(200 cells/μl500 cells/μl)three groups.The Tfh cells expressed CD4+ CXCR5 + ICOS+ and lymphocyte subpopulation including T,B and NK cells were detected by flow cytometry (FCM).The expression of Bcl-6 mRNA levels were detected by reverse transcription-polymerase chain reaction (RT-PCR).The association between Tfh cells and Bcl-6 mRNA,B cells were analyzed.Results The expression of Bcl-6 mRNA from patients of A,B and C three groups was increased than in healthy individuals (2.94±0.91,2.27±0.62,2.15± 0.351,P <0.05).The percent of Tfh cells from group A patients was higher than group C patients (5.88±3.01 vs 1.26± 0.87,P =0.032)and also the healthy control group (5.88±3.01 vs 0.78±0.42,P =0.004).The percentage of NK cells and B cells in HIV/AIDS patients was no statistically significant.Correlation analysis showed that there was positive corre-lation between the proportion of Tfh cells and Bcl-6 mRNA (r=0.799,P =0.000).And Tfh cells were observed no relation-ship with B cells (r=-0.083,P =0.657).Conclusion The changes of Bcl-6 mRNA and Tfh cells may be related to the progression of HIV/AIDS.
