Elevated Serum miR-193a-3p Level in Renal Clear Cell Carcinoma and Its Early Clinical Value
10.3969/j.issn.1671-7414.2015.03.012
- VernacularTitle:肾透明细胞癌患者血清miR-193a-3p 水平增加及早期诊断价值
- Author:
Haixia TANG
;
Cheng WANG
;
Meiling LU
;
Chunni ZHANG
- Publication Type:Journal Article
- Keywords:
renal clear cell carcinoma;
serum;
miR-193a-3p;
quantitativereverse-transcription PCR;
early diagnosis;
molecular biomarker
- From:
Journal of Modern Laboratory Medicine
2015;(3):43-45,49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the levels of miR-193a-3p in serum from patients with renal clear cell carcinoma,and eval-uate the potential of miR-193a-3p asa molecular biomarker of renal clear cell carcinoma.Methods Serum samples were taken from 107 untreated hospitalized patients with renal clear cell carcinoma (TNM Ⅰ grade 76 cases,Ⅱ grade 16 cases,Ⅲ grade 2 cases,Ⅳ grade 8 cases and unknown 5 cases)in Nanjing General Hospital from 2010 to 2014 year and 107 age-and gender-matched healthy individuals to determine the level of miR-193a-3p by quantitative reverse-transcription PCR (qRT-PCR). The early diagnostic value of miR-193a-3p for renal clear cell carcinoma was assessed by ROC curve.Results qRT-PCR confirmed that serum miR-193a-3p expression levels was significantly elevated in cancer than in normal controls (3.294± 1.526 vs 1.944±0.600,P =0.000).TNM Ⅰ grade (3.411±1.676 vs 1.944±0.600,P =0.000),Ⅱ grade (2.926±0.927 vs 1.944±0.600,P =0.001),Ⅳ grade(2.926±0.894 vs 1.944±0.600,P =0.000)is significantly higher than that in con-trol group.The area under the ROC curve (AUC)of miR-193a-3p was 0.820 (95% CI,0.756 ~ 0.883),demonstratinga high sensitivity (80.3%)and specificity (93.5%)for the early diagnosis of renal clear cell carcinoma.Conclusion MiR-193a-3p content was significantly elevated in serum from renal clear cell carcinoma and has a high accuracy in diagnosing ear-ly cancer,which holds potential as molecular biomarker for renal clear cell carcinoma.
