Establishing Physiological Body Fluid Method to Evaluate the Normal Value of Plasmic Free Amino Acid in Normal Healthy Volunteers
10.3969/j.issn.1671-7414.2015.01.013
- VernacularTitle:生理体液法测定40例健康志愿者血浆游离氨基酸正常值
- Author:
Guizhen HE
;
Zhibing TAO
;
Wenhui ZHANG
;
Jie WANG
- Publication Type:Journal Article
- Keywords:
amino acid determination;
amino acid normal value;
physiological fluid method
- From:
Journal of Modern Laboratory Medicine
2015;(1):46-51,54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the plasma amino acid levels and evaluate the free amino acids value of normal healthy volun-teers in plasma by establishing physiological fluid method.Methods ①Establishment of methodology:including repeatabili-ty of blood sample,recovery rate,linearity,and stability;②The free amino acid values in plasma in 40 normal healthy volun-teers were analyzed.Results With the standard solution of different concentrations (2,4 and 8 nmol)analyzed and linear re-gression of amino acid calculated,found that R2 of all the amino acids were within the range from 1 to 0.9 9 9.The repeatabili-ty with in batch and between batches of different amino acid in blood samples were respectively 0.30~2.90 (rsd)and 0.40~5.16(rsd);recovery rates were 93.1%~107.4%.Then evaluated the normal values of plasma amino acid in 40 healthy volunteers (male vs female:1 vs 1 ),and compared the results with that measured by other instruments.Conclusion With lithium citrate buffer system to analyze the free amino acid in blood samples,it displayed good reproducibility,recovery rate, linearity and stability.Comparison of different instruments measuring the free amino acids in plasma of normal healthy vol-unteers showed good agreement,confirming that the method of physiological fluid analysis is reliable.
