Research on Pseudomonas Aeruginosa Quinolone-resistant Plasmid Genes
10.3969/j.issn.1671-7414.2015.01.011
- VernacularTitle:铜绿假单胞菌对耐喹诺酮药物质粒基因的研究
- Author:
Shike LUO
;
Xianhua LIU
;
Pingan ZHU
;
Chunhui FAN
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
quinolone;
plasmid;
gene
- From:
Journal of Modern Laboratory Medicine
2015;(1):39-41,45
- CountryChina
- Language:Chinese
-
Abstract:
Objective Pseudomonasaeruginosa resistance to quinolones and around symbiotic bacteria resistant plasmids each chromosome metastasis.Methods 481 samples was collected from the Seventh People’s Hospital of Shenzhen since January 2011 to December 2013,and it cultured Pseudomonasaeruginosa 31 cases,susceptibility testing confirmed 15 cases of quin-olone-resistant as the research obj ect,using plasmid transformation,pick up experiments confirmed the presence of the re-sistance plasmid,PCR amplification,gene sequence analysis,and gene sequences surrounding the symbiotic bacteria resistant plasmids as a same.Results The same gene sequence of plasmid was found between drug-resistant strains of Pseudomonas aeruginosa and the surrounding symbiotic bacteria[χ2=1.207,P<0.01].Conclusion Resistance plasmids could be trans-ferred between different species of bacteria.
