Study on Proliferation of Pancreatic Cancer SW1990 Cell Lines by Small Interfering RNA Targeted Apollon
10.3969/j.issn.1671-7414.2015.01.008
- VernacularTitle:Apollon siRNA抑制胰腺癌SW1990细胞增殖的研究
- Author:
Xiang TANG
;
Li WANG
;
Zeping HAN
;
Jinhua HE
;
Baoxia LI
- Publication Type:Journal Article
- Keywords:
Apollon;
inhibitor of apoptosis proteins;
small interfering RNA;
pancreatic cancer;
SW1990 cell
- From:
Journal of Modern Laboratory Medicine
2015;(1):27-30,33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of small interfering RNA targeted on Apollon for proliferation on pancreatic cancer cells and its possible acting mechanism.Methods The small interfering RNA targeted on apollon in our previous study was transfected to the cells using LipofectamineTM 2000,after 48 hours transtection.The inhibitory effects of small interfering RNA targeted on Apollon (Apollon siRNA)on cell proliferation were detected by WST-8.Their inhibition rate and IC50 were calculated.The percentage of apoptosis cells were determined by flow cytometry.The expression of Apollon mRNA was analyzed by real time fluorescent quantitative reverse transcription polymerase chain reaction.The Apollon protein ex-pression levels were detected by western blotting.Results Apollon siRNA could effectively inhibit the proliferation of pan-creatic cancer cell.The amount of apoptotic cells increased significantly.The early apoptotic rate was 37.1%,and the RT-PCR results showed that the relative expression levels of Apollon mRNA were down-regulate,and shows a dose-effective-ness relations.The protein expression levels were decreased by Apollon siRNA.Conclusion Apollon siRNA can effectively inhibit the proliferation of pancreatic cancer cell.The mechanism may be work together to promote pancreative cancer cell early apoptosis and decreased the expression levels of gene and protein,which provides a novel potential approach for treat-ment of target therapy of pancreatic cancer.
