Comparison and Analysis of Three Methods in Testing Low Concentration HBsAg Samples

VernacularTitle:三种方法检测低浓度乙肝表面抗原的比较与分析
Author: Ming CHEN ; Qun SHEN ; Yu LIU ; Richu LIANG
Publication Type:Journal Article
Keywords: low concentration hepatitis B surface antigen; enzyme-linked immunosorbent assay; chemiluminescence microparti-cle immunoassay; time-resolved fluorescence immunoassay
From: Journal of Modern Laboratory Medicine 2014;(6):107-109,112
CountryChina
Language:Chinese
Abstract: Objective To make a preliminary study on the results from qualitative and quantitative detection of low concentra-tion hepatitis B surface antigen(HBsAg).Methods 85 HBsAg low concentration serum samples (ELISA method test re-sults were 0.60.05)and 0.60.05).The positive detection rate showed statistically difference between ELISA (70.6%)and CMIA,TRFIA in 0.6TRFIA>ELISA.②The contents of HBsAg showed statistically difference among 0.6TRFIA>ELISA.③There was a positive correlation between the three methods of HBsAg content (t=2.939,2.928,60.915,P<0.05).The correlation between CMIA method and TRFIA method was the best (r=0.989).Con-clusion CMIA was the first choice for testing low concentration HBsAg,TRFIA was the second.For the specimens of the low concentration HBsAg detected by ELISA should be suggested to clinical and retested by CMIA or TRFIA in order to a-void missing detection.And it was not recommended to clinical that different methods of quantitative or half-quantitative re-sults were transverse compared in order to avoid misdiagnosis.