Study on the Mechanism of Trimethoprim-Sulfamethoxazole Resistance in Stenotrophomonas Maltophilia
10.3969/j.issn.1671-7414.2014.06.008
- VernacularTitle:嗜麦芽窄食单胞菌对复方新诺明耐药的机制研究
- Author:
Suying ZHAO
;
Hongxiang LIU
;
Min LI
;
Huiling CAO
;
Hengfang SHI
- Publication Type:Journal Article
- Keywords:
stenotrophomonas maltophilia;
trimethoprim-sulfamethoxazole(TMP-SMZ);
intI 1;
qacE△1-sul1;
sul2
- From:
Journal of Modern Laboratory Medicine
2014;(6):25-27,31
- CountryChina
- Language:Chinese
-
Abstract:
Objective Class 1 integrase(intI 1),qacE△1-sul1 and sul2 genes were detected in trimethoprim-sulfamethoxazole resistant Stenotrophomonas maltophilia by PCR to assess the relationship between the antibiotic resistance mechanism for trimethoprim-sulfamethoxazole (TMP-SMZ)and these genes distribution.Methods S.maltophilia isolates were collected from patients treated in Affilitated hospital of Nanjing University of TCM during January to May in 2013,DNA was ab-stracted by boiling method and genes were detected by polymerase chain reaction.Results intI 1 genes were observed posi-tive in 25 of 28 strains resistant for TMP-SMZ,qacE△1-sul1 genes were positive in 21 while sul2 genes positive in 15,the positive rates of intI 1,qacE△1-sul1 and sul2 genes were 89.29%,75% and 53.57%;in 18 trimethoprim-sulfamethoxazole sensitive strains,5 were intI 1 positive,4 were qacE△1-sul1 positive while sul2 were none,the positive rates of intI1 and qacE△1-sul1 were 27.78% and 22.22%.Conclusion Most of stenotrophomonas maltophilia resisted trimethoprim-sulfa-methoxazole had intI 1,qacEΔ1-sul 1 and sul2 genes.
