Discovery of Human Leukocyte Antigen HLA-A/C Gene Recombination

VernacularTitle:人类白细胞抗原HLA-A／C座位间基因重组的发现
Author: Zhongmei WANG ; Na LIU ; Zhiyin GONG ; Lijun WANG ; Wei LI ; Xiaoyan SHAN
Publication Type:Journal Article
Keywords: HLA antigens; gene recombination; HLA-A/C loci
From: Journal of Modern Laboratory Medicine 2014;(5):107-108,111
CountryChina
Language:Chinese
Abstract: Objective To investigate the recombination events occurring between HLA-A and-C loci discovered in two Chi-nese families.Methods HLA class I (HLA-A and-B)and II (HLA-DRB1)alleles low resolution typing were typed by pol-ymerase chain reaction-sequence specific oligonucleotide,(PCR-SSO)and PCR-sequence specific primer (PCR-SSP).HLA class I and II high resolution typing was done by sequencing-based typing (SBT).Then the recombination sites were ana-lyzed by family study.Results The results indicates that the recombination events occurred in one family between A*30∶01/32∶01-C*06∶02 and another family between A*11∶01/26∶01-C*07∶06 during meiosis.The recombination both came from fathers and resulted in new HLA haplotypes that were inherited by the children.Conclusion Two HLA-A/C re-combination events occurring between HLA-A and-C loci have been found in two Chinese families,which help further study the mechanisms of HLA recombination.