Evaluation of Plasma miR-3 2 4-5 p for Early Diagnosis in Patients with Simple Congenital Heart Disease

VernacularTitle:血浆miR-324-5p对单纯性先天性心脏病早期诊断的应用评估
Author: Nan WAN ; Lu WANG ; Wei ZHENG ; Dongya MENG ; Xiaofang HU
Publication Type:Journal Article
Keywords: congenital heart disease; miR-324-5p; early diagnosis
From: Journal of Modern Laboratory Medicine 2014;(5):58-59,63
CountryChina
Language:Chinese
Abstract: Objective To analyze expression of miR-324-5p in plasma of CHD patients by real-time PCR and identification of early diagnosis,for CHD.Methods In 2012 June to 2013 February 76 patients and 13 normal controls who were included in the study had measurement of plasma expression levels of miR-324-5p by real-time PCR.MedCalc 13.0 software were used for statistical analysis and comprehensive evaluation of miR-324-5p in CHD disease for diagnosis.Results Analysis of re-ceiver operating curve (ROC)showed that area under the curve (AUC)was 0.731 and best diagnostic threshold was 0.116 1.The sensitivity was 64.5% and specificity of 84.6%.Conclusion Circulating miR-324-5p as a biomarker for early diagno-sis of CHD has some-extent clinical value,but needs combined with other medical indicators.