Effects of Skp2 overexpression on sensitivity of troglitazone in breast cancer cells
10.3969/j.issn.1673-4130.2015.07.001
- VernacularTitle:乳腺癌细胞中过表达S期激酶相关蛋白2对曲格列酮敏感性的影响
- Author:
Jie MENG
;
Yan ZOU
;
Fengxian QING
;
Xiaomou WEI
;
Guisheng WANG
;
Hao WU
;
Shengming DAI
- Publication Type:Journal Article
- Keywords:
skp2;
troglitazone;
breast cancer cells;
sensitivity
- From:
International Journal of Laboratory Medicine
2015;(7):865-866,869
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Skp2 overexpression on the sensitivity of troglitazone (TRG) in breast cancer cells and to devote to develop a novel drug for increasing the patient survival rate and eventually reaching the cure goal .Methods The transcription activities of PPARγ were analyzed on peroxisome proliferators response element(PPRE) luciferase reporter .The flow cytometry analysis and CCK‐8 assay were adopted to study that overexpression of Skp2 was associated with resistance to TRG‐mediated inhibition growth and apoptosis of breast cancer cells .Results Our study found that overexpression of Skp2 inhibi‐ted the transcriptional activity of the endogenous PPARγ and resisted to TRG‐mediated inhibition growth and apoptosis of breast cancer cells .Conclusion Overexpressed Skp2 breast cancer cells is able to be resistant to TRG‐induced sensitivity of breast cancer cells .Furthermore down‐regulating Skp2 will significantly enhance the growth inhibition of TRG on breast cancer cells .
