Determination of Ochratoxin A in Chinese Herbs by HPLC-MS/MS
- VernacularTitle:高效液相色谱-串联质谱法测定中药材中赭曲霉素A
- Author:
Ming DENG
;
Hao LI
;
Bin ZHU
- Publication Type:Journal Article
- Keywords:
Chineseherbs;
OchratoxinA;
HPLC-MS/MS;
Immunoaffinitycolumn
- From:
China Pharmacist
2014;(10):1652-1654,1655
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine ochratoxin A ( OTA ) in Chinese herbs by high performance liquid chromatography-tandem mass spectrometry method ( HPLC-MS/MS) . Methods: The samples were extracted by 80% methanol water solution and purified by immunoaffinity column. The chromatographic separation was carried out on a C18 column, the mobile phase was methanol-acetonitrile-0. 01 mol·L-1 ammonium acetate, and then OTA was detected by MS/MS in an ESI(-)-MRM mode. Results:The limit of detection was 0. 1 μg·kg-1 , the average recoveries ranged from 84. 8% to 91. 2%, and the relative standard deviations ( RSD) ranged from 3. 6% to 8. 1%(n=9). Conclusion:The method is accurate,sensitive and simple, and suitable for the determination of ochratoxin A in Chinese herbs.
